PrimePCR™ SYBR® Green Assay: TRPT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052729

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045468
Assay Design:   exonic
Chromosome Location:   11:63992324-63992413question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000542282 ENST00000394547 ENST00000317459 ENST00000546089 ENST00000545812 ENST00000394546 ENST00000541278 ENST00000254854 ENST00000377551 ENST00000377559 ENST00000409571 ENST00000265459 ENST00000382387 ENST00000453981 ENST00000432829 ENST00000328585

Gene Information

Description Not Available

Gene Symbol:   TRPT1
Gene Name:   tRNA phosphotransferase 1
Aliases:   MGC11134
RefSeq:   NC_000011.9 NG_016360.1 NT_167190.1
Ensembl:   ENSG00000149743
Entrez:   83707
UniGene:   Hs.326586
Chromosome Mapping:   11q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.290000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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