PrimePCR™ SYBR® Green Assay: CELF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052703

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045442
Assay Design:   exonic
Chromosome Location:   11:47510391-47510489question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000395290 ENST00000526419 ENST00000535982 ENST00000543178 ENST00000525841 ENST00000528434 ENST00000530151 ENST00000532048 ENST00000531165 ENST00000361904 ENST00000310513 ENST00000395292 ENST00000358597

Gene Information

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains one C-terminal RRM domain and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CELF1
Gene Name:   CUGBP, Elav-like family member 1
Aliases:   BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, NAB50, NAPOR, hNab50
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000149187
Entrez:   10658
UniGene:   Hs.595333
Chromosome Mapping:   11p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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