PrimePCR™ SYBR® Green Assay: PPRC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052689

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045428
Assay Design:   exonic
Chromosome Location:   10:103901447-103901536question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000278070 ENST00000370012 ENST00000413464

Gene Information

The protein encoded by this gene is similar to PPAR-gamma coactivator 1 (PPARGC1/PGC-1) a protein that can activate mitochondrial biogenesis in part through a direct interaction with nuclear respiratory factor 1 (NRF1). This protein has been shown to interact with NRF1. It is thought to be a functional relative of PPARGC1 that activates mitochondrial biogenesis through NRF1 in response to proliferative signals. [provided by RefSeq Jul 2008]

Gene Symbol:   PPRC1
Gene Name:   peroxisome proliferator-activated receptor gamma, coactivator-related 1
Aliases:   KIAA0595, MGC74642, PRC, RP11-302K17.6
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000148840
Entrez:   23082
UniGene:   Hs.533551
Chromosome Mapping:   10q24.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.870000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download