PrimePCR™ SYBR® Green Assay: INA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052687

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045426
Assay Design:   exonic
Chromosome Location:   10:105048610-105048722question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000369849

Gene Information

Neurofilaments are type IV intermediate filament heteropolymers composed of light medium and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq Jun 2009]

Gene Symbol:   INA
Gene Name:   internexin neuronal intermediate filament protein, alpha
Aliases:   FLJ18662, FLJ57501, MGC12702, NEF5, NF-66, TXBP-1
RefSeq:   NC_000010.10 NG_012318.1 NT_030059.13
Ensembl:   ENSG00000148798
Entrez:   9118
UniGene:   Hs.500916
Chromosome Mapping:   10q24.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.940000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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