PrimePCR™ SYBR® Green Assay: HABP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052684

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HABP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HABP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045423
Assay Design:   exonic
Chromosome Location:   10:115337888-115338465question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000351270 ENST00000541666 ENST00000542051 ENST00000537906

Gene Information

The protein encoded by this gene is an extracellular serine protease that binds hyaluronic acid and is involved in cell adhesion. The encoded protein is synthesized as a single chain but then undergoes an autoproteolytic event to form the functional heterodimer. Further autoproteolysis leads to smaller inactive peptides. This protease is known to cleave urinary plasminogen activator coagulation factor VII and the alpha and beta chains of fibrinogen but not prothrombin plasminogen or the gamma chain of fibrinogen. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2010]

Gene Symbol:   HABP2
Gene Name:   hyaluronan binding protein 2
Aliases:   FSAP, HABP, HGFAL, PHBP
RefSeq:   NC_000010.10 NT_030059.13 NG_008956.1
Ensembl:   ENSG00000148702
Entrez:   3026
Chromosome Mapping:   10q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.610000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download