This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052684
PrimePCR™ PreAmp for SYBR® Green Assay: HABP2, Human
PrimePCR™ Template for SYBR® Green Assay: HABP2, Human
The protein encoded by this gene is an extracellular serine protease that binds hyaluronic acid and is involved in cell adhesion. The encoded protein is synthesized as a single chain but then undergoes an autoproteolytic event to form the functional heterodimer. Further autoproteolysis leads to smaller inactive peptides. This protease is known to cleave urinary plasminogen activator coagulation factor VII and the alpha and beta chains of fibrinogen but not prothrombin plasminogen or the gamma chain of fibrinogen. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Apr 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.