PrimePCR™ SYBR® Green Assay: SH3GLB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052667

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045406
Assay Design:   exonic
Chromosome Location:   9:131784595-131790398question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000372564 ENST00000372559 ENST00000372554 ENST00000417224 ENST00000416629

Gene Information

Description Not Available

Gene Symbol:   SH3GLB2
Gene Name:   SH3-domain GRB2-like endophilin B2
Aliases:   KIAA1848, PP6569, PP9455
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000148341
Entrez:   56904
UniGene:   Hs.460238
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.230000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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