This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052640
PrimePCR™ PreAmp for SYBR® Green Assay: ADHFE1, Human
PrimePCR™ Template for SYBR® Green Assay: ADHFE1, Human
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 188.8.131.52) which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al. 2006 [PubMed 16616524]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.