PrimePCR™ SYBR® Green Assay: SH3KBP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052610

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045349
Assay Design:   exonic
Chromosome Location:   X:19606812-19606917question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000397821 ENST00000379716 ENST00000379698 ENST00000379726 ENST00000379697 ENST00000541422

Gene Information

This gene encodes an adapter protein that contains three N-terminal Src homology domains a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis cytoskeletal rearrangement cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:   SH3KBP1
Gene Name:   SH3-domain kinase binding protein 1
Aliases:   CD2BP3, CIN85, GIG10, HSB-1, HSB1, MIG18
RefSeq:   NC_000023.10 NT_167197.1 NG_021367.1
Ensembl:   ENSG00000147010
Entrez:   30011
UniGene:   Hs.726365
Chromosome Mapping:   Xp22.1-p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.100000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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