PrimePCR™ SYBR® Green Assay: CRIP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052575

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CRIP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CRIP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045314
Assay Design:   exonic
Chromosome Location:   6:43275365-43275478question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000250360 ENST00000222329 ENST00000389282 ENST00000576273 ENST00000592419 ENST00000436336 ENST00000451876 ENST00000292476 ENST00000216237 ENST00000445265 ENST00000323668 ENST00000439160 ENST00000513346 ENST00000451292 ENST00000377797 ENST00000263083 ENST00000406581 ENST00000223361 ENST00000452185 ENST00000433715 ENST00000456038 ENST00000418438 ENST00000457710 ENST00000440501 ENST00000321666 ENST00000430980 ENST00000329152 ENST00000377432 ENST00000164139 ENST00000505231 ENST00000435205 ENST

Gene Information

Description Not Available

Gene Symbol:   CRIP3
Gene Name:   cysteine-rich protein 3
Aliases:   TLP, TLP-A, bA480N24.2
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000146215
Entrez:   401262
Chromosome Mapping:   6p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 38.350000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download