PrimePCR™ SYBR® Green Assay: MLIP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052573

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MLIP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MLIP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045312
Assay Design:   exonic
Chromosome Location:   6:53989296-53989419question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000358276 ENST00000514433 ENST00000502396 ENST00000503951 ENST00000511678 ENST00000447836 ENST00000370876 ENST00000460844 ENST00000509997 ENST00000370877 ENST00000274897 ENST00000514921 ENST00000505762

Gene Information

Description Not Available

Gene Symbol:   MLIP
Gene Name:   muscular LMNA-interacting protein
Aliases:   C6orf142, FLJ54673, FLJ79056, MGC18257
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000146147
Entrez:   90523
Chromosome Mapping:   6p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download