PrimePCR™ SYBR® Green Assay: BDP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052546

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: BDP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: BDP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045285
Assay Design:   exonic
Chromosome Location:   5:70862744-70862892question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000573110 ENST00000358731 ENST00000380675

Gene Information

The product of this gene is a subunit of the TFIIIB transcription initiation complex which recruits RNA polymerase III to target promoters in order to initiate transcription. The encoded protein localizes to concentrated aggregates in the nucleus and is required for transcription from all three types of polymerase III promoters. It is phosphorylated by casein kinase II during mitosis resulting in its release from chromatin and suppression of polymerase III transcription. [provided by RefSeq Jul 2008]

Gene Symbol:   BDP1
Gene Name:   B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Aliases:   DKFZp686C01233, DKFZp686K0831, HSA238520, KIAA1241, KIAA1689, TAF3B1, TFC5, TFIIIB'', TFIIIB150, TFIIIB90, TFNR
RefSeq:   NC_000005.9 NT_006713.15 NW_003315917.1
Ensembl:   ENSG00000145734
Entrez:   55814
Chromosome Mapping:   5q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download