This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052546
PrimePCR™ PreAmp for SYBR® Green Assay: BDP1, Human
PrimePCR™ Template for SYBR® Green Assay: BDP1, Human
The product of this gene is a subunit of the TFIIIB transcription initiation complex which recruits RNA polymerase III to target promoters in order to initiate transcription. The encoded protein localizes to concentrated aggregates in the nucleus and is required for transcription from all three types of polymerase III promoters. It is phosphorylated by casein kinase II during mitosis resulting in its release from chromatin and suppression of polymerase III transcription. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.