PrimePCR™ SYBR® Green Assay: SSBP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052542

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045281
Assay Design:   exonic
Chromosome Location:   5:80716167-80716303question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000320672 ENST00000509053 ENST00000514493 ENST00000504985 ENST00000512923 ENST00000505980 ENST00000515395

Gene Information

SSBP2 is a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability (Huang et al. 2009) [PubMed 19683501].[supplied by OMIM Feb 2010]

Gene Symbol:   SSBP2
Gene Name:   single-stranded DNA binding protein 2
Aliases:   DKFZp686F03273, HSPC116, SOSS-B2
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000145687
Entrez:   23635
UniGene:   Hs.102735
Chromosome Mapping:   5q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.580000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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