This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052510
PrimePCR™ PreAmp for SYBR® Green Assay: DGKQ, Human
PrimePCR™ Template for SYBR® Green Assay: DGKQ, Human
The protein encoded by this gene contains three cysteine-rich domains a proline-rich region and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.