PrimePCR™ SYBR® Green Assay: DGKQ, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052510

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DGKQ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DGKQ, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045249
Assay Design:   exonic
Chromosome Location:   4:955617-956234question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000361355 ENST00000414303 ENST00000370083 ENST00000370080 ENST00000330330 ENST00000370079 ENST00000273814 ENST00000509465 ENST00000515182 ENST00000342992 ENST00000460472 ENST00000589042 ENST00000591111 ENST00000360870 ENST00000342175 ENST00000359218

Gene Information

The protein encoded by this gene contains three cysteine-rich domains a proline-rich region and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq Jul 2008]

Gene Symbol:   DGKQ
Gene Name:   diacylglycerol kinase, theta 110kDa
Aliases:   DAGK, DAGK4, DAGK7
RefSeq:   NC_000004.11 NT_037622.5
Ensembl:   ENSG00000145214
Entrez:   1609
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 35.040000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download