PrimePCR™ SYBR® Green Assay: MUC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052506

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045245
Assay Design:   exonic
Chromosome Location:   3:195498539-195498680question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000475231 ENST00000463781 ENST00000346145 ENST00000349607

Gene Information

The major constituents of mucus the viscous secretion that covers epithelial surfaces such as those in the trachea colon and cervix are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq Jul 2008]

Gene Symbol:   MUC4
Gene Name:   mucin 4, cell surface associated
Aliases:   HSA276359
RefSeq:   NC_000003.11 NT_029928.13
Ensembl:   ENSG00000145113
Entrez:   4585
UniGene:   Hs.369646
Chromosome Mapping:   3q29

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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