PrimePCR™ SYBR® Green Assay: MANF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052504

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045243
Assay Design:   exonic
Chromosome Location:   3:51426344-51426483question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000566546 ENST00000205890 ENST00000418233 ENST00000391745 ENST00000552699 ENST00000267115 ENST00000549385 ENST00000395006 ENST00000547798 ENST00000423828 ENST00000528157 ENST00000425354 ENST00000394426 ENST00000327550 ENST00000252992 ENST00000451429 ENST00000574138 ENST00000571761 ENST00000571164

Gene Information

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region including a specific mutation that changes the previously numbered codon 50 from ATG to AGG have been reported in a variety of solid tumors; however these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related. [provided by RefSeq Jun 2010]

Gene Symbol:   MANF
Gene Name:   mesencephalic astrocyte-derived neurotrophic factor
Aliases:   ARMET, ARP, MGC142148, MGC142150
RefSeq:   NC_000003.11 NG_012652.2 NG_028012.1 NT_022517.18 NW_003315910.1
Ensembl:   ENSG00000145050
Entrez:   7873
UniGene:   Hs.436446
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.690000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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