This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052504
PrimePCR™ PreAmp for SYBR® Green Assay: MANF, Human
PrimePCR™ Template for SYBR® Green Assay: MANF, Human
The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of polymorphisms in the arginine-rich region including a specific mutation that changes the previously numbered codon 50 from ATG to AGG have been reported in a variety of solid tumors; however these polymorphisms were later shown to exist in normal tissues and are thus not tumor-related. [provided by RefSeq Jun 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.