PrimePCR™ SYBR® Green Assay: EIF2A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052497

List Price:    $138.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045236
Assay Design:   exonic
Chromosome Location:   3:150289751-150289894question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000487799 ENST00000460851 ENST00000406576 ENST00000273435 ENST00000465535 ENST00000383043

Gene Information

EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al. 2002 [PubMed 12133843]).[supplied by OMIM Mar 2008]

Gene Symbol:   EIF2A
Gene Name:   eukaryotic translation initiation factor 2A, 65kDa
Aliases:   EIF-2A, MST089, MSTP004, MSTP089
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000144895
Entrez:   83939
UniGene:   Hs.655782
Chromosome Mapping:   3q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.990000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download