PrimePCR™ SYBR® Green Assay: NR1I2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0052491

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045230
Assay Design:   exonic
Chromosome Location:   3:119536593-119536718question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000393716 ENST00000466380 ENST00000337940

Gene Information

This gene product belongs to the nuclear receptor superfamily members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4 binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4 including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms some of which use non-AUG (CUG) translation initiation codon have been described for this gene. Additional transcript variants exist however they have not been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   NR1I2
Gene Name:   nuclear receptor subfamily 1, group I, member 2
Aliases:   BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR
RefSeq:   NC_000003.11 NG_011856.1 NT_005612.16
Ensembl:   ENSG00000144852
Entrez:   8856
UniGene:   Hs.7303
Chromosome Mapping:   3q12-q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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