This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052477
PrimePCR™ PreAmp for SYBR® Green Assay: SLC22A14, Human
PrimePCR™ Template for SYBR® Green Assay: SLC22A14, Human
This gene encodes a member of the organic-cation transporter family. It is located in a gene cluster with another member of the family organic cation transporter like 3. The encoded protein is a transmembrane protein which is thought to transport small molecules and since this protein is conserved among several species it is suggested to have a fundamental role in mammalian systems. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.