PrimePCR™ SYBR® Green Assay: METTL21A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052458

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: METTL21A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: METTL21A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045197
Assay Design:   exonic
Chromosome Location:   2:208488965-208489091question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000524918 ENST00000313028 ENST00000525773 ENST00000565818 ENST00000568154 ENST00000562335 ENST00000523500 ENST00000520796 ENST00000373377 ENST00000343450 ENST00000284727 ENST00000381318 ENST00000432416 ENST00000458426 ENST00000425132 ENST00000448007 ENST00000272839 ENST00000448823 ENST00000406927 ENST00000426075 ENST00000442521 ENST00000411432 ENST00000289388 ENST00000284006 ENST00000263388 ENST00000601011

Gene Information

Description Not Available

Gene Symbol:   METTL21A
Gene Name:   methyltransferase like 21A
Aliases:   FAM119A, HCA557b, MGC45373
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000144401
Entrez:   151194
Chromosome Mapping:   2q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.880000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download