This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052412
PrimePCR™ PreAmp for SYBR® Green Assay: ACTA1, Human
PrimePCR™ Template for SYBR® Green Assay: ACTA1, Human
The product encoded by this gene belongs to the actin family of proteins which are highly conserved proteins that play a role in cell motility structure and integrity. Alpha beta and gamma actin isoforms have been identified with alpha actins being a major constituent of the contractile apparatus while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3 congenital myopathy with excess of thin myofilaments congenital myopathy with cores and congenital myopathy with fiber-type disproportion diseases that lead to muscle fiber defects. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.