PrimePCR™ SYBR® Green Assay: RGS5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052368

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGS5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGS5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045107
Assay Design:   exonic
Chromosome Location:   1:163117193-163117289question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000433713 ENST00000431397 ENST00000548672 ENST00000425845 ENST00000372321 ENST00000448135 ENST00000429828 ENST00000602967 ENST00000429984 ENST00000396581 ENST00000376724 ENST00000437563 ENST00000552119 ENST00000383610 ENST00000547289 ENST00000434151 ENST00000549728 ENST00000313961 ENST00000367903 ENST00000530507 ENST00000527988 ENST00000430485

Gene Information

This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants have been identified. [provided by RefSeq Dec 2011]

Gene Symbol:   RGS5
Gene Name:   regulator of G-protein signaling 5
Aliases:   MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129
RefSeq:   NC_000001.10 NG_027731.1 NT_004487.19
Ensembl:   ENSG00000143248
Entrez:   8490
Chromosome Mapping:   1q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 36.550000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download