PrimePCR™ SYBR® Green Assay: BRP44, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052354

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045093
Assay Design:   exonic
Chromosome Location:   1:167887371-167887508question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000367846 ENST00000271373

Gene Information

Description Not Available

Gene Symbol:   BRP44
Gene Name:   brain protein 44
Aliases:   DKFZp564B167, MGC125752, MGC125753
RefSeq:   NC_000001.10 NG_016139.1 NT_004487.19
Ensembl:   ENSG00000143158
Entrez:   25874
UniGene:   Hs.517768
Chromosome Mapping:   1q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.760000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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