PrimePCR™ SYBR® Green Assay: PGD, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052335

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PGD, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PGD, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045074
Assay Design:   exonic
Chromosome Location:   1:10463182-10464327question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000523390 ENST00000522605 ENST00000221742 ENST00000600144 ENST00000598504 ENST00000544886 ENST00000448552 ENST00000306585 ENST00000356396 ENST00000359902 ENST00000301897 ENST00000353278 ENST00000358525 ENST00000328649 ENST00000371098 ENST00000218104 ENST00000327946 ENST00000356098 ENST00000216124 ENST00000395621 ENST00000453344 ENST00000395619 ENST00000547307 ENST00000547805 ENST00000413302 ENST00000401443 ENST00000336296 ENST00000402329 ENST00000443995 ENST00000382396 ENST00000397272 ENST

Gene Information

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq Jul 2008]

Gene Symbol:   PGD
Gene Name:   phosphogluconate dehydrogenase
Aliases:   6PGD
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000142657
Entrez:   5226
Chromosome Mapping:   1p36.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.130000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download