PrimePCR™ SYBR® Green Assay: NLRP12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052317

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045055
Assay Design:   exonic
Chromosome Location:   19:54297300-54299132question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000324134 ENST00000391775 ENST00000391773 ENST00000345770 ENST00000391772 ENST00000535162 ENST00000351894 ENST00000354278 ENST00000358661

Gene Information

This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein which contains an N-terminal pyrin domain a NACHT domain a NACHT-associated domain and a C-terminus leucine-rich repeat region functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Alternatively spliced transcript variants encoding distinct isoforms have been described but the full-length nature of some of these has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   NLRP12
Gene Name:   NLR family, pyrin domain containing 12
Aliases:   CLR19.3, FCAS2, NALP12, PAN6, PYPAF7, RNO, RNO2
RefSeq:   NC_000019.9 NT_011109.16 NG_008651.1
Ensembl:   ENSG00000142405
Entrez:   91662
UniGene:   Hs.631573
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.550000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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