PrimePCR™ SYBR® Green Assay: NFIC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052303

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045041
Assay Design:   exonic
Chromosome Location:   19:3452489-3452608question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000589123 ENST00000395111 ENST00000586919 ENST00000341919 ENST00000590282 ENST00000443272 ENST00000346156

Gene Information

The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   NFIC
Gene Name:   nuclear factor I/C (CCAAT-binding transcription factor)
Aliases:   CTF, CTF5, MGC20153, NF-I, NFI
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000141905
Entrez:   4782
UniGene:   Hs.170131
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 36.190000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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