PrimePCR™ SYBR® Green Assay: MAPK4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052295

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045033
Assay Design:   exonic
Chromosome Location:   18:48189625-48189763question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000400384 ENST00000592595 ENST00000588540 ENST00000576626 ENST00000587800 ENST00000587115

Gene Information

Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus where it phosphorylates nuclear targets. [provided by RefSeq Jul 2008]

Gene Symbol:   MAPK4
Gene Name:   mitogen-activated protein kinase 4
Aliases:   ERK3, Erk4, PRKM4, p63MAPK
RefSeq:   NC_000018.9 NT_010966.14
Ensembl:   ENSG00000141639
Entrez:   5596
UniGene:   Hs.433728
Chromosome Mapping:   18q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.190000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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