This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052284
PrimePCR™ PreAmp for SYBR® Green Assay: TP53, Human
PrimePCR™ Template for SYBR® Green Assay: TP53, Human
This gene encodes tumor protein p53 which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest apoptosis senescence DNA repair or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation DNA-binding and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms which can regulate p53 transcriptional activity. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.