PrimePCR™ SYBR® Green Assay: SLMO1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052270

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045008
Assay Design:   exonic
Chromosome Location:   18:12427220-12429363question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000590956 ENST00000336990 ENST00000589952 ENST00000592149 ENST00000587862 ENST00000587735 ENST00000440960 ENST00000355937 ENST00000371033

Gene Information

Description Not Available

Gene Symbol:   SLMO1
Gene Name:   slowmo homolog 1 (Drosophila)
Aliases:   C18orf43, DKFZp547K106, FLJ31484, HFL-EDDG1, MGC120170, PRELID3A
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000141391
Entrez:   10650
UniGene:   Hs.514718
Chromosome Mapping:   18p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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