PrimePCR™ SYBR® Green Assay: MYO19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052257

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MYO19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MYO19, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044995
Assay Design:   exonic
Chromosome Location:   17:34888374-34888489question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000328724 ENST00000350249 ENST00000334743 ENST00000422945 ENST00000414043 ENST00000361571 ENST00000572543 ENST00000381311 ENST00000348066 ENST00000358183 ENST00000431794 ENST00000268852 ENST00000586007 ENST00000544606 ENST00000585719 ENST00000589404 ENST00000586886 ENST00000585818 ENST00000318426 ENST00000565536 ENST00000565206 ENST00000563959 ENST00000565614 ENST00000566688 ENST00000561703

Gene Information

Description Not Available

Gene Symbol:   MYO19
Gene Name:   myosin XIX
Aliases:   FLJ22865, MYOHD1
RefSeq:   NC_000017.10 NT_010783.15 NW_003315949.1
Ensembl:   ENSG00000141140
Entrez:   80179
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.970000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download