PrimePCR™ SYBR® Green Assay: MAN2C1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052223

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAN2C1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAN2C1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044961
Assay Design:   exonic
Chromosome Location:   15:75658928-75659938question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000481290 ENST00000531214 ENST00000320230 ENST00000388726 ENST00000424778 ENST00000392650 ENST00000602922 ENST00000217320 ENST00000300527 ENST00000409416 ENST00000397763 ENST00000357838 ENST00000310645 ENST00000407373 ENST00000332585 ENST00000446658 ENST00000401475 ENST00000431368 ENST00000452656 ENST00000403222 ENST00000382310 ENST00000437268 ENST00000535268 ENST00000569482 ENST00000565683 ENST00000563622 ENST00000267978 ENST00000568374 ENST00000566256 ENST00000561615 ENST00000259235 ENST

Gene Information

Description Not Available

Gene Symbol:   MAN2C1
Gene Name:   mannosidase, alpha, class 2C, member 1
Aliases:   DKFZp686E23167, MAN6A8, MANA, MANA1, MGC87979
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000140400
Entrez:   4123
Chromosome Mapping:   15q11-q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.730000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download