PrimePCR™ SYBR® Green Assay: FBLN5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052210

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FBLN5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FBLN5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044948
Assay Design:   exonic
Chromosome Location:   14:92343892-92347648question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000267620 ENST00000342058 ENST00000556154 ENST00000554121

Gene Information

The protein encoded by this gene is a secreted extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However its expression is reinduced in balloon-injured vessels and atherosclerotic lesions notably in intimal vascular smooth muscle cells and endothelial cells. Therefore the protein encoded by this gene may play a role in vascular development and remodeling. Defects in this gene are a cause of autosomal dominant cutis laxa autosomal recessive cutis laxa type I (CL type I) and age-related macular degeneration type 3 (ARMD3). [provided by RefSeq Jul 2008]

Gene Symbol:   FBLN5
Gene Name:   fibulin 5
Aliases:   ARMD3, DANCE, EVEC, FIBL-5, FLJ90059, UP50
RefSeq:   NC_000014.8 NG_008254.1 NT_026437.12
Ensembl:   ENSG00000140092
Entrez:   10516
Chromosome Mapping:   14q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download