PrimePCR™ SYBR® Green Assay: SLC24A4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052209

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044947
Assay Design:   exonic
Chromosome Location:   14:92961872-92962004question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000393265 ENST00000531433 ENST00000532405 ENST00000525557 ENST00000298877 ENST00000351924

Gene Information

This gene encodes a member of the potassium-dependent sodium/calcium exchanger protein family. Alternative splicing results in multiple transcript variants.[provided by RefSeq Jul 2010]

Gene Symbol:   SLC24A4
Gene Name:   solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
Aliases:   FLJ38852, NCKX4, SHEP6, SLC24A2
RefSeq:   NC_000014.8 NT_026437.12 NG_023408.1
Ensembl:   ENSG00000140090
Entrez:   123041
UniGene:   Hs.385530
Chromosome Mapping:   14q32.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.990000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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