PrimePCR™ SYBR® Green Assay: NAA30, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052204

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044942
Assay Design:   exonic
Chromosome Location:   14:57876171-57876265question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000555166 ENST00000556492 ENST00000554703 ENST00000298406

Gene Information

Description Not Available

Gene Symbol:   NAA30
Gene Name:   N(alpha)-acetyltransferase 30, NatC catalytic subunit
Aliases:   C14orf35, FLJ35355, MAK3, MGC141884, MGC141885, Mak3p, NAT12
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000139977
Entrez:   122830
UniGene:   Hs.165465
Chromosome Mapping:   14q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.870000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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