PrimePCR™ SYBR® Green Assay: ZIC5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052198

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044936
Assay Design:   exonic
Chromosome Location:   13:100615747-100615896question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000267294

Gene Information

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development and have been associated X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to a gene encoding zinc finger protein of the cerebellum 2 a related family member on chromosome 13. [provided by RefSeq Jul 2008]

Gene Symbol:   ZIC5
Gene Name:   Zic family member 5 (odd-paired homolog, Drosophila)
Aliases:   Not Available
RefSeq:   NC_000013.10 NT_009952.14
Ensembl:   ENSG00000139800
Entrez:   85416
UniGene:   Hs.508570
Chromosome Mapping:   13q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.730000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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