PrimePCR™ SYBR® Green Assay: SBNO1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052191

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044929
Assay Design:   exonic
Chromosome Location:   12:123780451-123780547question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000602750 ENST00000602398 ENST00000420886 ENST00000267176

Gene Information

Description Not Available

Gene Symbol:   SBNO1
Gene Name:   strawberry notch homolog 1 (Drosophila)
Aliases:   FLJ10701, FLJ10833, FLJ14066, FLJ16176, FLJ35199, MOP3, Sno
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000139697
Entrez:   55206
UniGene:   Hs.7012
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.130000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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