PrimePCR™ SYBR® Green Assay: TMTC3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052168

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044906
Assay Design:   exonic
Chromosome Location:   12:88542100-88542244question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000551088 ENST00000266712 ENST00000549011

Gene Information

This gene encodes a protein that belongs to the transmembrane and tetratricopeptide repeat-containing protein family. [provided by RefSeq May 2010]

Gene Symbol:   TMTC3
Gene Name:   transmembrane and tetratricopeptide repeat containing 3
Aliases:   DKFZp686C0968, DKFZp686M1969, DKFZp686O22167, DKFZp686O2342, FLJ90492, SMILE
RefSeq:   NG_008417.1 NC_000012.11 NG_021187.1 NT_029419.12
Ensembl:   ENSG00000139324
Entrez:   160418
UniGene:   Hs.331268
Chromosome Mapping:   12q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.490000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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