This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052161
PrimePCR™ PreAmp for SYBR® Green Assay: ANP32D, Human
PrimePCR™ Template for SYBR® Green Assay: ANP32D, Human
Phosphoprotein 32 (PP32) is a tumor suppressor that can inhibit several types of cancers including prostate and breast cancers. The protein encoded by this gene is one of at least two proteins that are similar in amino acid sequence to PP32 and are part of the same acidic nuclear phosphoprotein gene family. However unlike PP32 the encoded protein is tumorigenic. The tumor suppressor function of PP32 has been localized to a 25 amino acid region that is absent in the protein encoded by this gene. This gene does not contain introns. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.