This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0052142
PrimePCR™ PreAmp for SYBR® Green Assay: PDE6H, Human
PrimePCR™ Template for SYBR® Green Assay: PDE6H, Human
This gene encodes the inhibitory (or gamma) subunit of the cone-specific cGMP phosphodiesterase which is a tetramer composed of two catalytic chains (alpha and beta) and two inhibitory chains (gamma). It is specifically expressed in the retina and is involved in the transmission and amplification of the visual signal. Mutations in this gene are associated with retinal cone dystrophy type 3A (RCD3A). [provided by RefSeq Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.