PrimePCR™ SYBR® Green Assay: AGPAT9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052118

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044856
Assay Design:   exonic
Chromosome Location:   4:84526339-84526488question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000395226 ENST00000264409

Gene Information

This gene encodes a member of the lysophosphatidic acid acyltransferase protein family. The encoded protein is an enzyme which catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Jan 2012]

Gene Symbol:   AGPAT9
Gene Name:   1-acylglycerol-3-phosphate O-acyltransferase 9
Aliases:   AGPAT8, GPAT3, LPAAT-theta, MAG1, MGC11324
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000138678
Entrez:   84803
UniGene:   Hs.99196
Chromosome Mapping:   4q21.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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