PrimePCR™ SYBR® Green Assay: ALS2CR8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052096

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ALS2CR8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ALS2CR8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044834
Assay Design:   exonic
Chromosome Location:   2:203782627-203782756question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000402905 ENST00000414490 ENST00000431787 ENST00000444724 ENST00000414857 ENST00000430899 ENST00000445120 ENST00000441569 ENST00000432024 ENST00000443740 ENST00000414439 ENST00000428585 ENST00000447539 ENST00000434998 ENST00000438828 ENST00000545253 ENST00000456821 ENST00000545262 ENST00000320443

Gene Information

Description Not Available

Gene Symbol:   ALS2CR8
Gene Name:   amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8
Aliases:   CARF, DKFZp667N246, FLJ12675, FLJ21579, NYD-SP24
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000138380
Entrez:   79800
Chromosome Mapping:   2q33.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.060000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download