PrimePCR™ SYBR® Green Assay: TIMM23, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052093

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044831
Assay Design:   exonic
Chromosome Location:   10:51592447-51592594question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000444743 ENST00000260867 ENST00000374065 ENST00000374064

Gene Information

Description Not Available

Gene Symbol:   TIMM23
Gene Name:   translocase of inner mitochondrial membrane 23 homolog (yeast)
Aliases:   FLJ40725, FLJ56773, FLJ57459, FLJ79448, MGC71995, MGC87383, TIM23
RefSeq:   NC_000010.10 NG_023372.1 NT_030059.13
Ensembl:   ENSG00000138297
Entrez:   100287932
Chromosome Mapping:   10q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.060000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download