PrimePCR™ SYBR® Green Assay: ACTR1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052078

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044816
Assay Design:   exonic
Chromosome Location:   10:104240475-104240618question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000369905 ENST00000487599 ENST00000446605 ENST00000545684

Gene Information

This gene encodes a 42.6 kD subunit of dynactin a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions including ER-to-Golgi transport the centripetal movement of lysosomes and endosomes spindle formation chromosome movement nuclear positioning and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule and is the most abundant molecule in the dynactin complex. It is an actin-related protein and is approximately 60% identical at the amino acid level to conventional actin. [provided by RefSeq Jul 2008]

Gene Symbol:   ACTR1A
Gene Name:   ARP1 actin-related protein 1 homolog A, centractin alpha (yeast)
Aliases:   ARP1, CTRN1, FLJ52695, FLJ52800, FLJ55002
RefSeq:   NC_000010.10 NG_011901.1 NG_021338.1 NT_030059.13
Ensembl:   ENSG00000138107
Entrez:   10121
UniGene:   Hs.153961
Chromosome Mapping:   10q24.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.570000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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