PrimePCR™ SYBR® Green Assay: UGGT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051973

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044711
Assay Design:   exonic
Chromosome Location:   2:128947423-128947515question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000259253 ENST00000418197 ENST00000375990

Gene Information

UDP-glucose:glycoprotein glucosyltransferase (UGT) is a soluble protein of the endoplasmic reticulum (ER) that selectively reglucosylates unfolded glycoproteins thus providing quality control for protein transport out of the ER.[supplied by OMIM Oct 2009]

Gene Symbol:   UGGT1
Gene Name:   UDP-glucose glycoprotein glucosyltransferase 1
Aliases:   FLJ21763, FLJ23671, FLJ23796, HUGT1, UGCGL1, UGT1
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000136731
Entrez:   56886
UniGene:   Hs.731708
Chromosome Mapping:   2q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.870000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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