PrimePCR™ SYBR® Green Assay: CKAP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051928

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CKAP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CKAP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044666
Assay Design:   exonic
Chromosome Location:   13:53035202-53035350question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000468284 ENST00000378034 ENST00000258607 ENST00000378037 ENST00000490903 ENST00000480747

Gene Information

CKAP2 is a cytoskeleton-associated protein involved in mitotic progression (Seki and Fang 2007 [PubMed 17376772]).[supplied by OMIM Mar 2008]

Gene Symbol:   CKAP2
Gene Name:   cytoskeleton associated protein 2
Aliases:   DKFZp686L1238, FLJ10749, LB1, TMAP, se20-10
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000136108
Entrez:   26586
Chromosome Mapping:   13q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.670000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download