This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051849
PrimePCR™ PreAmp for SYBR® Green Assay: CTSL1, Human
PrimePCR™ Template for SYBR® Green Assay: CTSL1, Human
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin as well as alpha-1 protease inhibitor a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes including myofibril necrosis in myopathies and in myocardial ischemia and in the renal tubular response to proteinuria. This protein which is a member of the peptidase C1 family is a dimer composed of disulfide-linked heavy and light chains both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Apr 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.