This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051838
PrimePCR™ PreAmp for SYBR® Green Assay: COL4A2, Human
PrimePCR™ Template for SYBR® Green Assay: COL4A2, Human
This gene encodes one of the six subunits of type IV collagen the major structural component of basement membranes. The C-terminal portion of the protein known as canstatin is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.