PrimePCR™ SYBR® Green Assay: SLC43A3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051834

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: SLC43A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC43A3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044572
Assay Design:   exonic
Chromosome Location:   11:57193668-57193801question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000395123 ENST00000395124 ENST00000352187 ENST00000529554 ENST00000533524 ENST00000530005 ENST00000525474 ENST00000529112 ENST00000528187 ENST00000529494 ENST00000533245 ENST00000533235 ENST00000524863 ENST00000532795 ENST00000533051 ENST00000529896 ENST00000526621 ENST00000530316 ENST00000529748 ENST00000532278 ENST00000448023 ENST00000359128 ENST00000301749 ENST00000419350 ENST00000308873 ENST00000399916

Gene Information

Description Not Available

Gene Symbol:   SLC43A3
Gene Name:   solute carrier family 43, member 3
Aliases:   DKFZp762A227, EEG1, FOAP-13, PRO1659, SEEEG-1
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000134802
Entrez:   29015
Chromosome Mapping:   11q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download