This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0051829
PrimePCR™ PreAmp for SYBR® Green Assay: HOOK1, Human
PrimePCR™ Template for SYBR® Green Assay: HOOK1, Human
This gene encodes a member of the hook family of coiled-coil proteins which bind to microtubules and organelles through their N- and C-terminal domains respectively. The encoded protein localizes to discrete punctuate subcellular structures and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.