PrimePCR™ SYBR® Green Assay: PRH2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051823

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRH2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044561
Assay Design:   exonic
Chromosome Location:   12:11082903-11083374question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000400707 ENST00000340184 ENST00000231229 ENST00000568259 ENST00000563379 ENST00000567310 ENST00000573223 ENST00000572835 ENST00000389811 ENST00000439055 ENST00000373991 ENST00000380358 ENST00000358181 ENST00000330494 ENST00000439235 ENST00000440097 ENST00000273317 ENST00000370551 ENST00000370550 ENST00000544177 ENST00000357132 ENST00000358656 ENST00000532158 ENST00000532205 ENST00000454097 ENST00000418203 ENST00000349129 ENST00000369739 ENST00000237163 ENST00000428168 ENST00000381847 ENST

Gene Information

Description Not Available

Gene Symbol:   PRH2
Gene Name:   proline-rich protein HaeIII subfamily 2
Aliases:   DKFZp686B01256, DKFZp686F14256, DKFZp686I11251, DKFZp686J06255, DKFZp686L01253, DKFZp686L16244, DKFZp686M04243, DKFZp686N24248, PRH1, Pr
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000134551
Entrez:   5555
Chromosome Mapping:   12p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.470000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download