PrimePCR™ SYBR® Green Assay: IL15RA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0051820

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IL15RA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IL15RA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0044558
Assay Design:   exonic
Chromosome Location:   10:5998352-6001740question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000368460 ENST00000528354 ENST00000447291 ENST00000532039 ENST00000397250 ENST00000379971 ENST00000530685 ENST00000397255 ENST00000397246 ENST00000397251 ENST00000397248 ENST00000525219 ENST00000435171 ENST00000379977

Gene Information

This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA IL15RA is capable of binding IL15 with high affinity independent of other subunits which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.[provided by RefSeq Apr 2010]

Gene Symbol:   IL15RA
Gene Name:   interleukin 15 receptor, alpha
Aliases:   MGC104179
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000134470
Entrez:   3601
Chromosome Mapping:   10p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.780000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download